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Molecular Biology
A Guide to the NGS Workflow
NGS Library Preparation and Sample Prep
KAPA Pure Beads
KAPA Pure Beads from Roche
Be the first to write a review!
Citations:
(236)
Supplier Page
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Roche for
KAPA Pure Beads
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Product Specs
Item
KAPA Pure Beads
Company
Roche
Price
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Catalog Number
07983280001
Quantity
30 mL
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Roche
Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road
Indianapolis
,
Indiana
United States
Phone
: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911
Company Profile
Website:
sequencing.roche.com
Citations (236)
(1)
Hybrid-seq deciphers the complex transcriptional profile of the human BRCA1 DNA repair associated gene
RNA Biology
December 31, 2023
Panagiotis G. Adamopoulos, Konstantina Athanasopoulou, Michaela A. Boti, Georgios Dimitroulis, Glykeria N. Daneva, Panagiotis Tsiakanikas, Andreas Scorilas
selection (target fragment ~ 400bp) was followed to enrich the library for the desired fragments, using the
KAPA Pure Beads
(
Kapa Biosystems
Inc.) in the recommended dsDNA: beads ratio. In the next step, the quantification of
(2)
A high-quality genome for the slender anole (Anolis apletophallus): an emerging model for field studies of tropical ecology and evolution
G3: Genes, Genomes, Genetics
December 29, 2023
Renata M Pirani, Carlos F Arias, Kristin Charles, Albert K Chung, John David Curlis, Daniel J Nicholson, Marta Vargas, Christian L Cox, W Owen McMillan, Michael L Logan
phenol–chloroform method (Barnett and Larson 2012). We purified the extracted DNA with 3× concentration of
KAPA Pure Beads
(
Roche
Sequencing
) and quantified the purity using a
Qubit fluorometer
(
Thermo Fisher Scientific
). We
(3)
Investigating the Human Intestinal DNA Virome and Predicting Disease-Associated Virus-Host Interactions in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS)
Int J Mol Sci
December 8, 2023
Shen-Yuan Hsieh, George M. Savva, Andrea Telatin, Sumeet K. Tiwari, Mohammad A. Tariq, Fiona Newberry, Katharine A. Seton, Catherine Booth, Amolak S. Bansal, Thomas Wileman,
et al.
of 95 °C for 10 s, 55 °C for 20 s, and 72 °C for 3 min. All libraries were then pooled, cleaned with
KAPA pure beads
(
Roche Diagnostics Ltd
., Hassocks, UK), and quantified using an Invitrogen™ Quant-iT dsDNA
assay
kit
(4)
In-Host HEV Quasispecies Evolution Shows the Limits of Mutagenic Antiviral Treatments
Int J Mol Sci
December 6, 2023
Sergi Colomer-Castell, Josep Gregori, Damir Garcia-Cehic, Mar Riveiro-Barciela, Maria Buti, Ariadna Rando-Segura, Judit Vico-Romero, Carolina Campos, Marta Ibañez-Lligoña, Caroline Melanie Adombi,
et al.
elongation temperature at 50 °C and 72 °C, respectively (Figure 7).
PCR product
was purified using
KAPA Pure Beads
(
Kapa Biosystems
, Roche, Pleasanton, CA, USA). Quantification was carried out by Qubit
fluorometry
using
(5)
Comparison of Nanopore and Synthesis-Based Next-Generation Sequencing Platforms for SARS-CoV-2 Variant Monitoring in Wastewater
Int J Mol Sci
December 6, 2023
David Garcia-Pedemonte, Albert Carcereny, Josep Gregori, Josep Quer, Damir Garcia-Cehic, Laura Guerrero, Adrià Ceretó-Massagué, Islem Abid, Albert Bosch, Maria Isabel Costafreda,
et al.
control mixtures and wastewater samples were purified. The RNA controls were purified with magnetic
Kapa Pure Beads
(
Roche
, Basel, Switzerland) and the wastewater samples with the QIAquick Gel Extraction Kit (Qiagen,
(6)
Protocol to capture transcription factor-mediated 3D chromatin interactions using affinity tag-based BL-HiChIP
STAR Protocols
December 1, 2023
Ruimin Ren, Yao Hua, Heng Wang
I-Block Protein-Based Blocking Reagent
,
Invitrogen
,Cat#
T2015
Dynabeads MyOne Streptavidin C1
,
Invitrogen
,Cat#
65001
KAPA pure beads
,
KAPA
,Cat#KK8001 ,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, Gel
(7)
Ecological and clinical evidence of the establishment of West Nile virus in a large urban area in Europe, Berlin, Germany, 2021 to 2022
Euro Surveill
December 1, 2023
Claudia Ruscher, Corinna Patzina-Mehling, Julia Melchert, Selina L Graff, Sarah E McFarland, Christian Hieke, Anne Kopp, Anita Prasser, Torsten Tonn, Michael Schmidt,
et al.
final extension step at 72 °C. Resulting
PCR
products were pooled and purified with a twofold volume of
KAPA Pure Beads
according to the manufacturer’s instructions (
Roche
). For library preparation, the
PCR
Sequencing
kit
(8)
Whole-Genome Sequencing of 5-Hydroxymethylcytosine at Base Resolution by Bisulfite-Free Single-Step Deamination with Engineered Cytosine Deaminase
ACS Central Science
November 30, 2023
Neng-Bin Xie, Min Wang, Wei Chen, Tong-Tong Ji, Xia Guo, Fang-Yin Gang, Ya-Feng Wang, Yu-Qi Feng, Yuan Liang, Weimin Ci,
et al.
a Hieff
NGS
Ultima
DNA Ligation
Module Kit (Yeasen), and the resulting DNA was purified using 0.8×
KAPA Pure beads
(
Roche
). To the resulting mixture were added DNA-L-5mC and DNA-5hmC as spike-ins to evaluate the deamination
(9)
Species-specific gill’s microbiome of eight crab species with different breathing adaptations
Scientific Reports
November 29, 2023
Giovanni Bacci, Niccolò Meriggi, Christine L. Y. Cheng, Ka Hei Ng, Alessio Iannucci, Alessio Mengoni, Duccio Cavalieri, Stefano Cannicci, Sara Fratini
Enzyme.
PCR
products were checked through
electrophoresis
on 1.5% agarose gel and then purified using
KAPA Pure Beads
(
Roche
) following the manufacturer’s instructions. The indexing step was performed with 2 ×
KAPA HiFi HotStart ReadyMix
(10)
Spatial profiling of cancer-associated fibroblasts of sporadic early onset colon cancer microenvironment
npj Precision Oncology
November 14, 2023
Satoru Furuhashi, Matias A. Bustos, Shodai Mizuno, Suyeon Ryu, Yalda Naeini, Anton J. Bilchik, Dave S. B. Hoon
in equal amounts. Pooled library was purified twice with a 1.2X magnetic beads-to-sample ratio with
KAPA Pure Beads
(
Roche
, Basel, Switzerland), each followed by two 80% ethanol washes, then eluted with Elution Buffer
(11)
Gut Microbiota Signatures Are Associated With Psychopathological Profiles in Patients With Ulcerative Colitis: Results From an Italian Tertiary IBD Center
Inflammatory Bowel Diseases
November 2, 2023
Franco Scaldaferri, Antonio Maria D’Onofrio, Rosaria Calia, Federica Di Vincenzo, Gaspare Filippo Ferrajoli, Valentina Petito, Eleonora Maggio, Pia Clara Pafundi, Daniele Napolitano, Letizia Masi,
et al.
seconds, at 72°C for 30 seconds, and a final step at 72°C for 5 minutes. DNA amplicons were cleaned up by
KAPA Pure Beads
(
Roche Diagnostics
, Mannheim, Germany). Indexed libraries were obtained by using Nextera technology
(12)
Canine diffuse large B-cell lymphoma downregulates the activity of CD8 + T-cells through tumor-derived extracellular vesicles
Cancer Cell International
October 26, 2023
Hsin-Pei Weng, Chiao-Hsu Ke, Chun-Wei Tung, Akiyoshi Tani, Chia-Chi Wang, Wen-Yuan Yang, Yu-Shan Wang, Winston Han, Chi-Hsun Liao, Hirotaka Tomiyasu,
et al.
that appropriate adapter sequences were at both ends with
library amplification
primers. Finally, the
KAPA Pure Beads
system (
KAPA Biosystems
) was used to purify
PCR
products, and the library quality was estimated with
(13)
Investigating Antibody Reactivity to the Intestinal Microbiome in Severe Myalgic Encephalomyelitis/Chronic Fatigue Syndrome (ME/CFS): A Feasibility Study
International Journal of Molecular Sciences
October 18, 2023
Katharine A. Seton, Marianne Defernez, Andrea Telatin, Sumeet K. Tiwari, George M. Savva, Antonietta Hayhoe, Alistair Noble, Ana L. S. de Carvalho-KoK, Steve A. James, Amolak Bansal,
et al.
to 50 min. DNA was precipitated using 0.7X solid phase reversible immobilisation bead
clean-up
with
KAPA pure beads
(
Roche
, Basel, Switzerland).
Whole genome amplification
was performed using the REPLI-g advanced DNA
(14)
Multiple Sclerosis-Associated Gut Microbiome in the Israeli Diverse Populations: Associations with Ethnicity, Gender, Disability Status, Vitamin D Levels, and Mediterranean Diet
International Journal of Molecular Sciences
October 9, 2023
Zehavit Nitzan, Elsebeth Staun-Ram, Anat Volkowich, Ariel Miller
San Francisco, CA, USA) were used to add adaptor and index sequences, and the reactions cleaned using
Kapa Pure beads
(
Kapa
, Roche, Basel, Switzerland). The concentration of each library was measured by Qubit (Life Technologies,
(15)
Comparison of Antibacterial Activity of Phytochemicals against Common Foodborne Pathogens and Potential for Selection of Resistance
Microorganisms
October 5, 2023
Ryan Sweet, Catherine Booth, Kathryn Gotts, Stephen F. Grove, Paul A. Kroon, Mark Webber
recently [30]. Briefly,
cultured cells
were lysed and DNA was isolated via DNA-binding magnetic beads (
KAPA Pure Beads
,
Roche Diagnostics
) and eluted with 10 mM Tris-Cl, pH 8.5. The extracted DNA samples were sequenced
(16)
Characterisation of neonatal Staphylococcus capitis NRCS-A isolates compared with non NRCS-A Staphylococcus capitis from neonates and adults
Microbial Genomics
October 4, 2023
Heather Felgate, Dheeraj Sethi, Kirsten Faust, Cemsid Kiy, Christoph Härtel, Jan Rupp, Rebecca Clifford, Rachael Dean, Catherine Tremlett, John Wain,
et al.
GloMax Discover Microplate Reader
before being pooled and double-SPRI size selected between 0.5 and 0.7X bead volumes using
KAPA Pure Beads
(
Roche
). The final pool was quantified on a Qubit 3.0 instrument and run on a
D5000 ScreenTape
(
Agilent
)
(17)
Single-Nucleotide Resolution Mapping of N6-Methyladenine in Genomic DNA
ACS Central Science
September 27, 2023
Cheng-Jie Ma, Gaojie Li, Wen-Xuan Shao, Yi-Hao Min, Ping Wang, Jiang-Hui Ding, Neng-Bin Xie, Min Wang, Feng Tang, Yu-Qi Feng,
et al.
recommended
procedures
. The extracted DNA was eluted in 200 μL of H2O and further enriched by 0.4×
KAPA Pure Beads
(
Roche
). The obtained DNA was stored at −20 °C until use. Enzymatic Digestion of DNA A 30 μL mixture
(18)
Uncoupling the TFIIH Core and Kinase Modules Leads To Unregulated RNA Polymerase II CTD Serine 5 Phosphorylation
bioRxiv
September 16, 2023
Gabriela Giordano, Robin Buratowski, Célia Jeronimo, Christian Poitras, François Robert, Stephen Buratowski
libraries. All ChIP and Input samples were subjected to a 1.7X cleanup using
KAPA Pure Beads
(
Roche
,
07983280001
) according to the manufacturer’s instructions. Samples were eluted with 40 μL of Elution buffer (10
(19)
Role of mucin glycosylation in the gut microbiota-brain axis of core 3 O-glycan deficient mice
Sci Rep
August 26, 2023
Erika Coletto, George M. Savva, Dimitrios Latousakis, Matthew Pontifex, Emmanuelle H. Crost, Laura Vaux, Andrea Telatin, Kirk Bergstrom, David Vauzour, Nathalie Juge
for 30 s, 55 °C for 30 s and 72 °C for 30 s with a final step at 72 °C for 5 min. A 0.7× SPRI using
KAPA Pure Beads
(
Roche
, UK) was then performed and the DNA was eluted in 20 μL of EB (10 mM Tris–HCl). Following the
(20)
Dynamics of Amino Acid Metabolism, Gene Expression, and Circulomics in a Recombinant Chinese Hamster Ovary Cell Line Adapted to Moderate and High Levels of Extracellular Lactate
Genes (Basel)
August 2, 2023
Dylan G. Chitwood, Lisa Uy, Wanfang Fu, Stephanie R. Klaubert, Sarah W. Harcum, Christopher A. Saski
starting material for a Phi-29-mediated
rolling circle amplification (RCA
) and magnetic bead
purification
(
KAPA Pure Beads
,
Roche
, KK8000) as described in the CIDER-seq protocol [37]. EccDNA libraries had SMRTbell barcodes
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KAPA Pure Beads from Roche
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